WASHING WITH ALKALINE SOLUTIONS IN PROTEIN A PURIFICATION IMPROVES PHYSICOCHEMICAL PROPERTIES OF MONOCLONAL ANTIBODIES

Washing with alkaline solutions in protein A purification improves physicochemical properties of monoclonal antibodies

Abstract Protein A affinity chromatography has been widely used for both laboratory scale purification and commercial manufacturing of monoclonal antibodies and Fc-fusion proteins.Protein A purification is specific and efficient.However, there still remain several issues to be addressed, such as incomplete clearance of impurities including host cel

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Journey of Limonene as an Antimicrobial Agent

Injudicious consumption of antibiotics in the past few decades has arisen the problem of resistance in pathogenic organisms against most antibiotics and antimicrobial agents.Scenarios of treatment failure are becoming more common in hospitals.This situation demands the frequent need for new antimicrobial compounds which may have other mechanisms of

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Detecting of Barely Visible Impact Damage on Carbon Fiber Reinforced Polymer Using Diffusion Ultrasonic Improved by Time-Frequency Domain Disturbance Sensitive Zone

Based on the decorrelation calculation of diffusion ultrasound in time-frequency domain, this paper discusses the repeatability and potential significance of Disturbance Sensitive Zone (DSZ) in time-frequency domain.The experimental study of Barely Visible Impact Damage (BVID) on Carbon Fiber Reinforced Polymer (CFRP) is carried out.The decorrelati

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